產品詳情
  • 產品名稱:Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(MW25000)

  • 產品型號:FSF0001
  • 產品廠商:FuShen
  • 產品價格:0
  • 折扣價格:0
  • 產品文檔:
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簡單介紹:
Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(MW25000)聚乙烯亞胺(Polyethylenimine)Max—高效線性PEI:可用作轉染試劑,也可用于**劑包被。Polyethylenimine "Max"與普通的線性化Polyethylenimine多聚物骨架相同。高效線性PEI含有更多的自由基(質子數),比普通PEI高11%。重復的乙烯亞胺單元平均數量約為581,因此,顯著提高了質子化氮的長度。CAS號:9002-98-6 分子式:(C2H5N)x Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(MW25000)Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(MW25000)聚乙烯亞胺(Polyethylenimine)Max—高效線性PEI:可用作轉染試劑,也可用于**劑包被。Polyethylen
詳情介紹:

Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(MW25000)

產品簡介:

中文名稱:PEI轉染試劑 ,線性聚乙烯亞胺

英文名稱:Polyethylenimine Linear(PEI)

產品貨號 產品名稱 規格  報價(元) 品牌
FSF0001-1ML        線性PEI(MW25000)轉染試劑溶液 1ml        180.00  FuShen進口分裝
FSF0001-10ML 線性PEI(MW25000)轉染試劑溶液                                                   10ml 480.00  FuShen進口分裝
FSF0001-50ML 線性PEI(MW25000)轉染試劑溶液 50ml 790.00  FuShen進口分裝
FSF0001-1G Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(MW25000) 1g 1450.00  FuShen進口分裝
FSF0001-2G Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(MW25000) 2g 2450.00 FuShen進口分裝
23966-1
Polyethylenimine, Linear (MW 25,000)
1g
2864.00 Polysciences原裝
FSF0002-1ML 線性PEI(速溶型MW40000)轉染試劑溶液 1ml 210.00  FuShen進口分裝
FSF0002-10ML 線性PEI(速溶型MW40000)轉染試劑溶液 10ml 540.00  FuShen進口分裝
FSF0002-50ML 線性PEI(速溶型MW40000)轉染試劑溶液 50ml 840.00  FuShen進口分裝
24765-1
Polyethylenimine “Max”, (nominally Mw 40,000*) - High Potency Linear PEI (Equivalent to Mw 25,000 in Free Base Form)
1g 3184.00 Polysciences原裝
FSF0002-1G Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(速溶型MW40000) 1g 1650.00  FuShen進口分裝
FSF0002-2G Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(速溶型MW40000) 2g 3050.00 FuShen進口分裝


      線性化聚乙烯亞胺PEI 25,000,一種高電荷陽離子聚合物,非常容易結合于高電荷陰離子基質。工業應用上,線性化PEI可改善負電荷染料的物理外觀,以調整染料的化學特性和增強染料與固相基質的黏附能力,常用作黏附增強劑,作用同多聚賴氨酸(poly-lysine);科研應用上,線性化PEI能與DNA或其他負電荷生物大分子簡單結合,正是這一特性,使得成為一種非常行之有效的載體運輸介質(Vector carrier),即轉染試劑。線性PEI轉染試劑是一種陽離子聚合物,分子量25000,它能與核酸形成復合物,并使該復合 物進入哺乳動物細胞。線性PEI轉染試劑廣泛適用于常見細胞系,如 HEK-293、HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。該試劑即使在有血清存在的情況下, 它仍然能高效的將核酸導入細胞。線性PEI轉染試劑具有優越的轉染效率 , 重組蛋白的高表達水平 ,與含血清的培養基相兼容, 低細胞毒性,易于操作 !產品應用:

Polyethylenimine “Max”, (nominally Mw 40,000*) - High Potency Linear PEI (Equivalent to Mw 25,000 in Free Base Form)高轉化效率的PEI Max 40,000,自由基形式分子量相當于PEI 25,000,研究證實其體外質粒DNA轉染效率提高達21倍。

產品屬性:

CAS:9002-98-6, 26913-06-4

分子式:(CH2CH2NH)n

熔點:73-75℃

溶解性:溶于熱水、低pH冷水, 甲醇和乙醇。不溶于:乙醚、丙酮和冷水。

外觀:白色到黃色固體

儲存:RT室溫

使用方法【來源于:CSH protocols,僅作交流學習】 :

1. Pour ~450 mL of Milli-Q H2O into a 500-mL glass beaker.

2. Add 500 mg of PEI to the beaker with stirring.

3. Add concentrated HCl drop-wise to the solution to pH <2.0.

4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH <2.0 throughout.

Approximately 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.

5. Add concentrated NaOH drop-wise to the solution to pH 7.0.

Approximately 500 μL of 10 M NaOH will be required to neutralize the PEI solution.

6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H2O.

7. Filter-sterilize the solution through a 0.22-μm membrane.

8. Store aliquots of the desired volume at -20°C.

注意事項

1)為了您的**和健康,請穿實驗服并帶一次性手套操作。

Reference(s)參考文獻:

Siegman S, Truong NF, Segura T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta Biomater. in Press, doi:10.1016/j.actbio.2015.09.020

Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002

Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A.102(16):5679-84.

Durocher, Y., Perret, S. & Kamen, A. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research 30, E9 (2002). doi:10.1093/nar/30.2.e9

Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(MW25000)Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(MW25000)Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(MW25000)Polyethylenimine Linear(PEI)線性化聚乙烯亞胺(MW25000)

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